Label-Free Imaging of Myelination in Adult Zebrafish Using Photoacoustic Microscopy

Photoacoustic microscopy (PAM) uses laser excitation and ultrasonic detection to identify different types of biological tissue. It produces label-free images of the specimen that is being examined. The cost-effective system in our lab uses PAM optics to identify the lipid-rich tissue areas and has been previously tested with drosophila larvae. However, we are working towards testing more complex organism such as zebrafish, a common organism used for research in demyelination. In this report, we will explore the results of adult zebrafish brain imaging with different wavelengths and compare the differences between that of the normal adult zebrafish and gold zebrafish to gather more information about the imagery contents.