Antibacterial resistance in the United States is a pressing issue that continues to grow. This can be credited to an overprescription of antibiotics in duality with a lack of consistency when taking said antibiotics. Overall, random mutation leads to bacteria that are resistant to antibiotics. Most antibiotics target either gram positive or gram negative bacteria and metal containing antibiotics have garnered some success. Generally these antibiotic agents are non-specific to the type of cell membrane of the bacteria. Currently research efforts in the Tyler Lab are aimed at synthesizing transition metal complexes that target both gram positive and gram negative bacteria. Prior to testing new metallodrugs, it is imperative that the correct methodology for these assays is developed. Toward this goal, I will be presenting the initial results using agar well diffusion methods with previously reported copper complexes and ampicillin, both of which inhibit the growth of gram negative E. coli. These studies will be compared to the new complexes. Additional methods will be reported that elucidate the concentration dependent activity of the complexes. Overall these efforts will enable us to adjust the parameters for the antibacterial studies in order to employ a broader range of transition metal complexes and ascertain their biological activity.