Ganoderma is a commonly found and farmed genus of mushrooms, native to Europe as well as parts of China, and is also used for medicinal purposes. The LFC1 gene encodes a transcription factor that has been shown to act as a negative regulator of the growth and development of the fruiting body (mushroom) of the related fungus Flammulina velutipes. A "knockout" or null allele of LFC1 results in an increase in the vegetative growth rate of that fungus. We have identified a likely LFC1 orthologue in Ganoderma, and are employing the gene-editing technique of CRISPR to knock out the gene to explore gene function. To this end, a Donor DNA recombinant DNA construct has been created for use in Homology Directed Repair (HDR). Experiments are proceeding to knock out the LFC1 gene, and if successful, we will compare the growth phenotype of this null mutant to that of the unedited Ganoderma strain.