Staphylococcus aureus is a circular shaped, gram-positive bacterium that has become a prominent pathogen in medical settings due to its ability to induce infection of the skin and underlying tissues. Approximately one-third of the U.S. population naturally harbors this organism in their nasal passages, but are typically asymptomatic. However, carriers have the potential to transmit the bacterium to other individuals through direct contact, most often via contaminated hands. Several studies have shown that a substantial number of healthcare workers test positive for this bacterium, thus illuminating the significant role it plays in healthcare settings. Additionally, the emergence of antibiotic resistant strains causes thousands of hospitalized patients to acquire MRSA (methicillin-resistant S. aureus) each year, illustrating the pervasiveness of this pathogen in medical settings. My study aimed to identify carriers of S. aureus among healthcare and non-healthcare workers. In addition, positive isolates were tested against a multitude of antibiotics to identify potential MRSA strains. One hundred volunteers were asked to swab the inside of their nostrils, providing a bacterial sample that was then grown and tested on selective and differential growth media, including mannitol salt, SM110, and blood agars. Positive results, along with the confirmation of grape-like morphology and arrangement through gram staining and coagulase activity, were used to identify strains of S. aureus. These cultures were then streaked on Mueller-Hinton agar to determine their susceptibility to eight antibiotics, including two cell wall-specific drugs, oxacillin (a methicillin equivalent) and penicillin. Our initial findings indicate that 21 (29.5%) out of 71 completely tested samples were identified as S. aureus strains, and 6 (28.6%) of the S. aureus strains were triply resistant to erythromycin, oxacillin, and penicillin.