The unique permeation channel and sp2 bonded carbon structure of graphene oxide (GO) membranes make them ideal for applications like filtration and biosensing. Radiofrequency (RF) plasma is used to change the properties and morphology of surfaces by concentrating ionized air over them. Plasma treating these membranes has shown to improve their hydrophilicity and efficiency by increasing pore size, introducing more oxygen-based functional groups to its surface, and etching the surface to provide a more uniform filter. The impact of RF air plasma on the wetting properties and vapor permeation performance of GO materials was studied. To understand the effect of modification, further structure and morphology modification is carried out in this study. Scanning electron microscopy (SEM) images will be utilized to examine visual changes in the membranes before and after treatment, while atomic force microscopy (AFM) provides quantitative data on the topographical changes on the membrane surface. Furthermore, the X-ray diffraction spectrum was applied to identify the interlayer structure change during plasma treatment, and the FTIR spectrum was used to understand the bonding structure variation during plasma treatment. Our results demonstrate that RF air plasma treatment is a facile and effective strategy to improve the permeation performance and antifouling behavior of GO materials.