We implement, from scratch a confocal laser-scanning microscope (CLSM) system applicable for Physics & Biology related projects ranging from the characterization and modulation of 2D material-based devices to in-vivo imaging of cellular structures and 3D sample reconstruction. Scanning microscopy offers several advantages over traditional wide-field fluorescence microscopy including: increased image resolution via the elimination of out-of-focus secondary fluorescence, the ability to resolve thick samples through a series of optical sections, and localized excitation. The device is built from readily available commercial products and implements multiple laser wavelengths, a 3-axis computer-controlled sample stage, broadband illumination, and spectroscopy capabilities. We provide an open-source, custom Python GUI-based software package written using the National Instruments API (NI-DAQmx) and additional ThorLabs driver-specific APIs. This system is capable of acquiring images in all coordinate planes of both biological and solid-state systems and provides opportunities for localized PL characterization, tiling, and other imaging methods.
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