DNA and RNA are large biomolecules that are essential to all known forms of life. Nucleic acids are composed of nucleotides, which are naturally occurring and serve as primary information carrying molecules in cells. The function of RNA in the body is diverse and broad, playing a role in regulating different genes and other biological processes through countless mechanisms. Under the broad classification of RNA, there is a family of RNAs called microRNAs. MicroRNAs (miRNAs) are noncoding RNAs that act as post-transcriptional gene regulators. MiRNAs are transcribed from the genome into large stem-loop structures that are known as primary miRNAs or pri-miRNAs. In the nucleus, pri-miRNAs are processed by two main enzymes into shorter stem loop precursors or pre-miRNAs. Another enzyme, Dicer, is able to recognize and cleave the stem loop structure to produce the mature miRNAs which then go on to regulate protein expression. In some cases, the pre-miRNA can fold into an alternative structure known as a G-quadruplex. Dicer is unable to cleave the G-quadruplex conformation of these pre-miRNA, inhibiting formation of the miRNA. The stem-loop and G-quadruplex conformations of pre-miRNAs are of interest to scientists because of their applications in cancer research. Certain miRNAs have been identified in the formation of cancer cells, as the mature miRNA can lead to the downregulation of tumor suppressant proteins. My research has been focused on small organic molecules that bind to the G-quadruplex conformation of pre-let-7e to stabilize the G-quadruplex, not allowing the miRNA to mature or downregulate the target proteins of let-7e. These compounds were identified as hit compounds for their selective binding to a fluorescently labeled pre-let-7e RNA through a technique known as small molecule microarray screening. I have been analyzing the binding of these molecules to the wild type and mutant RNA with data collected by steady state fluorimeter titrations. Through these titrations, we have identified molecules that bind to the pre-let-7e RNA in a dose-dependent fashion. Currently, I am using circular dichroism to better understand and analyze the structure of the wild type and mutant RNAs. Looking more closely at the structure of the different conformations will help us understand better why some molecules are binding to both the wild type and mutant forms of the RNA, rather than binding selectively as desired.